Abstract: In order to screen potential probiotics from pig intestines, the microbes in the feces of healthy Chenghua pigs were isolated and purified. According to in vitro bacteriostatic test, a strain with antibacterial effect was selected. Morphological observation, biochemical assays, 16 S rRNA PCR amplification and sequencing were carried out on the strain, and the measured sequence was analyzed by genetic evolutionary. Then acid and pig bile salt tolerance tests and drug sensitivity tests were carried out. The results showed that a strain of Enterococcus, which had inhibitory effect on Escherichia coli, Staphylococcus aureus and Streptococcus suis, was isolated and identified as Enterococcus durans by biochemical identification and PCR, named LE-Z. The survival rate of the strain reached 39.64% and 58.91% in the pH value 2.0 and 4.0 environment for 5 hours, respectively, and 56.73% and 12.82% in the environment with pig bile salt concentration of 0.25% and 0.5% for 2 hours. LE-Z was sensitive to most antibiotics, but resistant to five kinds of antibiotics of amikacin, ceftazidime, gentamicin, kanamycin and neomycin. These results indicated that strain LE-Z had certain drug resistance and could tolerate the environment of low pH value and high pig bile salt concentration to a certain extent. It can be used as a probiotic candidate for subsequent tests.