Abstract: In order to investigate the effect of different genomic arrangement positions of Rabies virus G protein on its virulence, the full-length recombinant plasmid of Rabies virus SRV9 rearrangement G protein was sequenced and identified by enzyme digestion. The purified full-length rearranged plasmid and its helper plasmid were co-transfected into BSR cells by reverse genetic technique to save the rearranged virus. Then the fifth generation of rearranged virus was detected by RT-PCR and indirect immuno fluorescence. The results showed that the rearranged full-length plasmid was completely correct in the connection sites of each gene. There were 3 mutations in L gene, and a small number of cells in BSR cells produced fluorescence after transfection. The rearranged virus fragments were 1 589 bp, 1 473 bp, 812 bp and 3 823 bp in size by RT-PCR, which was consistent with the expected results. Under a confocal microscope, the rearranged virus produced a large amount of green fluorescence. The results indicated that SRV9 strains of G protein rearrangement virus were successfully obtained by liposome transfection technique.