Abstract: Bovine tuberculosis is a zoonotic disease mainly caused by Mycobacterium tuberculosis variant bovis. Countries usually adopt "quarantine-culling" measures to reduce the economic losses caused by bovine tuberculosis and to ensure public health security. This article summarized some research results of bovine tuberculosis detection methods based on pathogen level, immune protein（and its derivatives） level, and nucleic acid（molecular） level, in order to provide theoretical basis for bovine tuberculosis quarantine and clinical diagnosis.