Abstract: In order to improve the detection method of Salmonella in live virus vaccine, this study explored the effect of enrichment on the detection rate, compared and screened Salmonella identification medium, verified the improved serological method, and established a molecular biology method. The results showed that the detection rate could be improved by bacteria enrichment. SS agar and HE agar were suitable for detection. In serological methods, it was feasible to replace O-multivalent-one Salmonella factor serum with A-F groups Salmonella specific diagnostic serum. The established molecular biological method could be used to finally identify the contaminated bacteria in live virus vaccine. The results suggested that the improved detection method could increase the detection rate of Salmonella, reduce the false positive rate, and could also identify the species of other contaminated bacteria