Abstract: In order to analyze the sequence of VP2 protein of Israeli acute paralysis virus（IAPV） LN strain and to predict the B lymphcyte antigenic epitope, in the experiment, the IAPV-LN strain RNA was used as a template, the VP2 gene was amplified by RT-PCR,the nucleotide sequence of the IAPV-LN strain VP2 gene was obtained through BLAST sequence alignment, and the amino acid sequence was deduced to establish a 3 D model of the VP2 protein. The Protein module in DNAStar software was used to comprehensively analyze the hydrophilicity, flexible area, surface possibility and antigenic epitope of VP2 protein. The results showed that the VP2 gene sequence of the IAPV-LN strain was 810 bp in length and 270 amino acids had been edited, which had a nucleotide sequence homology of 99% with the IAPV-BJ strain published in GenBank（GenBank accession number KX421583.1）,and the amino acid sequence homology was 99%;the nucleotide sequence homology with the IAPV-BJ2（GenBank accession number MG599488.1） strain was 98%,and the amino acid sequence homology was 99%. It was hydrophilie protein, flexible area and surface possibility were more. The spatial conformation of the VP2 protein of the IAPV-LN strain was relatively regular, and the regions where it may form an epitope were at amino acids 14-22,167-175,and 239-246.