Abstract: In order to understand the pathogen types and prevalence of calf diarrhea in Henan Province, in the experiment, 136 fecal samples of calves with diarrhea of different ages were collected in the Central, Northern, Southern, Eastern, and Western Henan Province. Antigen detection kits were used to detect four pathogens, Cryptosporidium parvum（C.parvum）, Bovine rotavirus（BRV）, Bovine coronavirus（BCoV）, and Escherichia coli K99⁺（E.coli K99⁺）. The pathogenic infections of different calf ages, fecal scores, and distribution areas were counted, and the distribution of single infection and mixed infection of the four pathogens was analyzed. The results showed that the infection rates of C.parvum, BRV, BCoV, and E.coli K99⁺ were 21.32%, 13.97%, 4.41%, and 2.94%, respectively. The detection rates of E.coli K99⁺ were significantly different between ≤six weeks of age and ＞six weeks of age（P＜0.05）; the detection rates of C.parvum, BCoV, BRV had no significant difference（P＞0.05）. For the detection rate of BRV, calves with a stool score of two were significantly higher than calves with a stool score of one（P＜0.05）. For the detection rate of E.coli K99⁺, calves with a stool score of three were significantly higher than calves with a stool score of one（P＜0.05）. It was found that the detection rates of the three types of mixed infections were not significantly different among different stool scores（P＞0.05）, and the detection rate of single infection and mixed infection was not significantly different among calves in different regions（P＞0.05）. The results suggested that the calves had serious infections of C.parvum and BRV in Henan Province.