Abstract: In order to understand the genetic variation of porcine Senecavirus（SVA） A in Guangdong area, the experiment employed virus isolation and purification, indirect immunofluorescence, proliferation kinetics, electron microscope observation and other methods for the determination of the infection of suspected disease materials. At the same time, the sequence alignment analysis method was used to analyze the homology with the representative whole genome sequences（SVA） in GenBank, and the MEGA6.0 software was used to construct the whole genome phylogenetic tree. The results showed that the isolated virus could react specifically with the SVA polyclonal antibody, and the virus particles were spherical with a diameter of 25-30 nm. A SVA strain was successfully isolated and named as CH-GDZQ-1. The virus titer of CH-GDZQ-1 strain reached the peak at the 15^th hour of infection of PK15 cells, which was 1×10^8.78 TCID₅₀/mL. The whole genome sequence of CH-GDZQ-1 had 98.1% nucleotide similarity with USA/GBI29/2015 strain, which had a high degree of homology. The strain was in the same evolutionary branch with the Chinese strains CH-GDLZ01-2017（MG428680）, CH-GDLZ02-2017（MG428681）, CH-GDQC-2017（MG428682）, CH-GDYD-2017（MG428683）, CH-GDYS01-2017（MG428684） and CH-GDYS02-2017（MG428685）, and was also in the same branch with the American strain USA/GBI29/2015（KT827251）. The results suggested that a USA/GBI29/2015-like strain was successfully isolated, which had a high virus titer, and the USA/GBI29/2015-like strain was prevalent in Guangdong area.