Abstract: In December 2020, the pregnant sows in a large-scale pig farm in Anhui Province suffered from diarrhea and abortion, which were suspected to be porcine epidemic diarrhea in clinical examination. In order to further diagnose the disease, in the experiment, primers were designed according to the gene fragments of four common viruses that caused diarrhea in pigs（Porcine epidemic diarrhea virus, Transmissible gastroenteritis virus, Porcine rotavirus, and Porcine kobuvirus）; small intestine samples of pregnant and aborted piglets were collected and total RNA was extracted for diagnosis by RT-PCR method. In addition, various internal organs such as stomach, duodenum, lymph node, kidney, heart, spleen, liver, tonsil of aborted piglets on 30 and 60 days of gestation were collected for histopathological examination. The results showed that a specific gene fragment was amplified from the RNA extracted from 10 small intestine samples of aborted piglets, which was consistent with the size of the target gene band of Porcine epidemic diarrhea virus. Pathological section observation showed that the gastric mucosal layer was hyperemia and edema, and the duodenal mucosa was detached, hyperemia and hemorrhage, which were consistent with the pathological changes caused by porcine epidemic diarrhea. The results suggested that the pregnant sows in this pig farm had been infected with Porcine epidemic diarrhea virus.