Abstract: An 11-year-old Miniature Pinscher showed a mung bean-sized growth in the upper right eyelid about four years ago; it gradually grew up later, and nearly half a month before the treatment, the dog’s scratching caused the epidermal ulceration of the proliferation, with blood scab. The diagnosis was carried out through medical history investigation, pathological examination, blood test and cytological analysis, and the treatment was performed based on the diagnosis results. The results showed that the dog was diagnosed as mastocytoma; the treatment method of surgical resection and skin transplantation had achieved good results. There was no recurrence and other abnormalities in the follow-up visit one year after the operation. The results suggested that the surgical treatment plan had a good therapeutic effect on this case.