Abstract: In order to understand the species of ticks that parasitized the surface of cattle in some areas of Tibet, the samples of ticks were collected in this experiment from Nyingchi and Lhasa areas, Tibet. Firstly the collected ticks were identified by morphological methods; then based on the 12 S rDNA, 16 S rDNA and COXⅠ genes of tick mitochondria, the sequences were amplified and sequenced; lastly the MEGA 7.0 software was used to construct a phylogenetic tree and perform molecular evolution analysis with the Neighbor-joining method, which was verified with the morphological results. The results showed that adult ticks collected in Nyingchi area, Tibet, belonged to Haemaphysalis through preliminary identification of morphology; adult ticks collected in Lhasa area, Tibet, belonged to Dermacentor. The nucleotide similarities between the COXⅠ, 12 S rDNA and 16 S rDNA gene sequences of the Haemaphysalis collected in Nyingchi area, Tibet and the known Haemaphysalis formosensis, Haemaphysalis longicornis, and Haemaphysalis danieli in GenBank were only 87.36%, 91.28%, and 90.31%, respectively. While the nucleotide similarities between the COXⅠ, 12 S rDNA and 16 S rDNA gene sequences of the Dermacentor collected in Nyingchi area, Tibet and the known Dermacentor everestianus in GenBank were 98.88%, 98.76% and 97.70%, respectively. The phylogenetic trees built according to different genes were different, but the gene sequences in the same genus could be clustered into one branch. The results suggested that the ticks collected in Lhasa area, Tibet in this experiment were Dermacentor everestianus; the ticks collected in Nyingchi area, Tibet had low nucleotide similarity with the existing ticks on GenBank, and the molecular evolutionary tree analysis indicated that the molecular evolutionary tree structure of different target genes was inconsistent; therefore, its specific species needed to be further studied.