Abstract: In order to understand the mutation and molecular evolution of VP2 gene of Senecavirus A（SVA） in Hebei Province, a pair of amplification primers were designed based on VP2 gene, RT-PCR was used to amplity and cloned for sequencing; the genetic evolution analysis of the VP2 gene sequence obtained by sequencing and the reference sequences from GenBank was performed by MEGA 7.0 software, and the nucleotide and amino acid homology analysis was performed by MegAlign 5.0 software. The results showed that the VP2 gene sequence was successfully amplified and sequenced, and the sequence was uploaded to GenBank, and the gene accession number was MZ031967, which was named SVA HB-BD strain. The nucleotide sequence similarity between the strain and the Chinese strains was 95.9%-99.6%, and its nucleotide similarity with the prototype strain SVV-001（DQ 64125） was 94.6%, which was at a low level, and the nucleotide similarity with other American strains（MN812946, MN812947, KC667560, MH704432, MN812938, MN812937, KU954086, NC011349） was 93.0%-94.6%. The SVA HB-BD strain was closely related to the isolates from 2015—2016, and they belonged to the same branch, while it was far from the isolates from 2017—2018. The amino acid sequence analysis showed that the SVA HB-BD strain had higher similarity with the isolated strains in Guangdong Province, and the neutralization epitope of VP2 protein was highly conserved. The results suggested that the HB-BD strain had the same origin as the Chinese strains and was constantly evolving.