Abstract: In order to study the immune effect of Canine parvovirus vaccine inoculated with needle-free syringe, in the experiment, 15 healthy Labrador Retrievers aged 42 to 45 days and 15 Golden Retrievers were randomly divided into 3 groups, 10 in each group（5 in each breed）. There was 1 head group with needle injection（containing Canine parvovirus antigen NL-35-D strain）, 1 head group with needle-free injection and 1/2 head group with needle-free injection. The 1 head group with needle injection was inoculated with 1 head of Weijiawu vaccine with a needle syringe, and the 1 head group with needle-free injection and the 1/2 head group with needle-free injection were inoculated with 1 and 1/2 head, respectively. On the 14 th and 28 th days after the first immunization, the second and third immunizations would be carried out in the same way. The clinical response of dogs was observed after immunization; blood was collected and serum was separated on the 14 th day after the first, second and third vaccinations, respectively. The antibody concentration was detected with Canine parvovirus（CPV Ab） enzyme-linked immunoassay（ELISA） kit, and the immune effect was judged according to the produced antibody concentration. The results showed that there were no inflammatory reactions and other adverse reactions in the dogs in the 1 and 1/2 head groups of needle-free injections, and the pain was significantly reduced. Antibody levels on the 14 th day after the second immunization of the experimental dogs in each group could achieve a protective effect. By comparison of the three groups on the 14 th day after the first, second, and third vaccinations, the antibody levels in the 1 and 1/2 head groups with needle-free injection were slightly higher than those in the 1 head group with needle injection, but the difference was not significant（P＞0.05）. The results suggested that Canine parvovirus vaccine immunization effect was reliable and safe when using needle-free syringe.