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缩合单宁对努比亚山羊生长性能、瘤胃液中挥发性脂肪酸含量及细菌菌群的影响

Effects of condensed tannin on growth performance,volatile fatty acid contents and bacterial flora in rumen fluid of Nubian goats

  • 摘要: 为了研究缩合单宁对努比亚山羊生长性能、瘤胃液中挥发性脂肪酸含量及细菌菌群的影响,试验将16只生长发育良好、平均体重为(25.78±1.79) kg的努比亚山羊随机均分为对照组和处理组,每组2个重复,每个重复4只,对照组饲喂基础日粮,处理组饲喂添加4%缩合单宁的试验日粮,预试期7 d,正试期50 d。试验结束后,分析两组试验羊只的生长性能指标(平均日增重、平均日采食量及料重比)差异,瘤胃液中挥发性脂肪酸(乙酸、丁酸、异丁酸、异戊酸、丙酸、戊酸)的含量、乙酸/丙酸(乙丙比)差异,以及瘤胃液中细菌菌群的差异。结果表明:处理组努比亚山羊的料重比显著低于对照组(P<0.05),乙酸、丁酸、异丁酸、异戊酸、丙酸、戊酸含量均显著低于对照组(P<0.05),乙丙比显著高于对照组(P<0.05),OUTs(5 925)低于对照组(6 906),Shannon指数、Chao1指数、Observedspecies指数均显著低于对照组(P<0.05)。在门水平,处理组努比亚山羊瘤胃液内拟杆菌门(Bacteroidetes)、未分类门(Bacteriaunclassified)、变形杆菌门(Proteobacteria)相对丰度较对照组分别降低了2.60%、11.15%、14.07%,而厚壁菌门(Firmicutes)相对丰度较对照组提高了19.22%。在属水平,处理组努比亚山羊瘤胃液内普雷沃菌属(Paraprevotella)相对丰度较对照组提高了33.36%,而拟杆菌科未分类菌属(Bacteroidalesunclassified)相对丰度较对照组降低了24.63%,未知属f型拟杆菌目RF16菌群(norank f Bacteroidales RF16 group)、瘤胃菌科未分类属(Ruminococcaceaeunclassified)、未分类属(Bacteriaunclassified)、理研菌属(Rikenella)、Succiniclasticum、紫单胞菌科未分类菌属(Porphyromonadaceaeunclassified)相对丰度均低于对照组。说明4%水平缩合单宁显著降低努比亚山羊料重比,抑制碳水化合物在努比亚山羊瘤胃内的发酵,对瘤胃内大部分细菌有抑制作用,但对厚壁菌门、普雷沃菌属有促进作用。

     

    Abstract: The objects of this experiment was to study the effects of condensed tannin on growth performace, volatile fattt acid contents and bacterial flora in rumen of Nubian goats, 16 Nubian goats with an average body weight of(25.78±1.79) kg with good growth were randomly divided into control group and treatment group with 2 replicates per group and 4 goats per replicate. Goats in the control group were fed with basal diet and treatment group were added 4% condensed tannin in the basal diet. The pre-feeding period lasted for 7 days, and the experiment lasted for 50 days. At the end of the experiment, the differences in growth performance indicators(average daily weight gain, average daily feed intake and feed-to-weight ratio), the content of volatile fatty acids(acetic acid, butyric acid, isobutyric acid, isovaleric acid, propionic acid and valeric acid) in rumen fluid, the ratio of acetic acid and propionic acid, and bacterial flora in rumen fluid were analyzed between the two groups of test sheep.The results showed that feed-to-weight ratio in treatment group was lower than that of control group(P<0.05). Acetic acid, butyric acid, isobutyric acid, isovaleric acid, propionic acid and valeric acid in control group were significantly higher than those in treatment group(P<0.05), and the ratio of acetic acid and propionic acid in treatment group was significantly higher than that in control group(P<0.05). OUTs(5 925) were lower than that in the control group(6 906). The Shannon index, Chao1 index and Observedspecies index in the treatment group were significantly lower than those in the control group(P<0.05). At the phylum level, compared with the control group, the relative abundance of Bacteroidetes, Bacteriaunclassified, and Proteobacteria in the rumen of Nubia goats in the treatment group decreased by 2.60%,11.15%, and 14.07%, respectively, and the relative abundance of Firmicutes increased by 19.22% compared with the control group. At the genus level, the relative abundance of Paraprevotella in the rumen of Nubian goats in the treatment group was 33.36% higher than that in the control group, while the relative abundance of Bacteroidalesunclassified in the treatment group was 24.63% lower than that in the control group. The abundance of norank f Bacteroidales RF16 group, Ruminococcaceaeunclassified, Bacteriaunclassified, Rikenella, Succiniclasticum, Porphyromonadaceaeunclassified of treatment group was lower than control group. The results indicated that 4% level of condensed tannin significantly reduced the feed-to-weight ratio of Nubia goats, inhibited carbohydrate fermentation in the rumen of Nubian goats, and had inhibitory effect on most bacteria in the rumen, but had promoting effect on Firmicutes and Prevotella.

     

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