Abstract: In order to explore the therapeutic effect and mechanism of berberine on carbon tetrachloride（CCl₄）-induced liver injury, in the experiment, 80 healthy female Kunming mice were selected, 40 of which were used for acute liver injury test and 40 for chronic liver injury test. The mice were randomly divided into control group, model group, berberine group and silybin group, with 10 mice in each group. In acute liver injury test, mice in berberine group and silybin group were pretreated with berberine solution（25 mg/kg, by body weight） and silybin solution（50 mg/kg, by body weight） every day for 7 days, and then orally administered 0.6% CCl₄（5 μL/g, by body weight） at one time. In the chronic liver injury test, 0.6% CCl₄ solution（5 μL/g, by body weight） was given intragastric administration twice a week for 6 weeks. After the chronic liver injury model was successfully established, berberine group and silybin group were given berberine solution（weight 50 mg/kg） and silybin solution（50 mg/kg） daily for 30 days. The mice with acute liver injury and chronic liver injury were killed 24 hours after the last administration, orbital blood was collected, and sera were collected to detect aspartate aminotransferase（AST） and alanine aminotransferase（ALT） activities; the liver was weighed wet and the liver index was calculated. The left lobe of the liver about 1 cm × 1 cm × 0.25 cm was taken and immersed in 4% paraformaldehyde buffer for histopathological examination; the remaining liver tissues were weighed and homogenized, and the activities of superoxide dismutase（SOD）, glutathione（GSH） and malondialdehyde（MDA） were detected. The results showed that in acute and chronic liver injury tests, compared with the model group, the liver index of berberine group was significantly decreased（P＜0.01）, and the pathological changes of liver tissue were significantly alleviated. In acute liver injury test, compared with the model group, the activities of AST and ALT in serum of berberine group were significantly decreased（P＜0.01）; the content of MDA in liver was significantly decreased（P＜0.05）, and the activities of SOD and GSH were significantly increased（P＜0.05）. In chronic liver injury test, compared with the model group, the serum AST activity in berberine group were significantly decreased（P＜0.01）; the ALT activity and MDA content were significantly decreased（P＜0.05）, and the activities of SOD and GSH in liver were significantly increased（P＜0.01）.The results suggested that berberine could reduce the degree of inflammation in mice with liver injury and significantly improve the tissue proliferation in mice with chronic liver injury.