Abstract: In order to understand the effect of selenizing Glycyrrhiza uralensis polysaccharide（SeGUP） on anti-inflammatory activity in chicks, the experiment used lipopolysaccharide（LPS） to establish an inflammation model in chicks. Three hundred 1-day-old Jinghong No.1 chicks were selected and randomly divided into five groups, namely the blank control group, the LPS group and the LPS + astragalus polysaccharide（APS） group, the LPS + Glycyrrhiza uralensis polysaccharide（GUP） group, the LPS + selenizing Glycyrrhiza uralensis polysaccharide（SeGUP） group. Except blank control group, after intraperitoneal injection of LPS in experimental chickens at 8, 10, and 12 days old, the LPS+APS group, the LPS+GUP group, and the LPS+SeGUP group were given APS, GUP, and SeGUP at a concentration of 1 mg/mL by instramuscular injection, respectively, 0.5 mL per chick for seven days. Ten chickens were chosen from each group at 18, 25, 32, 39, 46 days old. The phagocytic index of chicken macrophages was detected by carbon clearance method; the kits were used to detect the contents of tumor necrosis factor α（TNF-α）, interleukin 1β（IL-1β）, interleukin 2（IL-2）, interleukin 6（IL-6）, and nitric oxide（NO） in the serum of chicks and inducible NO synthase（iNOS）. The results showed that compared with the blank control group, 18, 25, 32, 39, 46 day-old chick macrophage phagocytic index and serum IL-1β, IL-2, IL-6, TNF-α, NO, iNOS levels were significantly increased in the LPS group（P＜0.05）; compared with the LPS group, 18, 25, 32, 39, 46 day-old chick macrophage phagocytic index and serum IL-1β, IL-2, IL-6, TNF-α, NO, iNOS levels were significantly decreased in the LPS+SeGUP group（P＜0.05）. The results suggested that SeGUP could alleviate the inflammatory response caused by LPS stimulation in chicks and played an anti-inflammatory effect.