Abstract: In order to solve the problem of low quality of frozen semen in sheep, semen from three 24-month-old healthy Dairy breeding rams was cryopreserved with Tris diluents containing 0,40, 80, 120,160 μmol/L resveratrol（RES） antioxidants, respectively. The sperm motility, plasma membrane integrity rate, acrosome integrity rate, sperm deformity rate and antioxidant capacity（malonaldehyde MDA content, glutathione peroxidaseGSH-Px activity and total antioxidant capacity T-AOC） of pre-and post-freezing sperm semen were measured and calculated to determine the optimal supplemental concentration of RES. The results showed as follows: the sperm motility, plasma membrane integrity rate and acrosome integrity rate in 80 μmol/L RES was the highest, the mean value were（61.36±3.45）%,（40.31±1.20）%,（54.08±2.27）%, respectively; and the sperm motility and plasma membrane integrity rate in 80 μmol/L RES were extremely significantly higher than that in 0,160 μmol/L RES（P＜0.01）,and were significantly higher than that in 40,120 μmol/L RES（P＜0.05）; acrosome integrity rate in 80 μmol/L RES was not significantly different from that in 40 μmol/L RES（P＞0.05）; it was extremely significantly higher than that in 160 μmol/L RES（P＜0.01）,and was significantly higher than that in 0,120 μmol/L RES（P＜0.05）.The sperm malformation rate and MDA content in 80 μmol/L RES were the lowest, the mean values were（14.27±0.69）% and（31.98±0.85）μmol/L, respectively; the sperm deformity rate in 80 μmol/L RES was not significantly different from that in 40 μmol/L RES（P＞0.05）,and was extremely significantly higher than that in 0,160 μmol/L RES（P＜0.01）, significantly higher than that in 120 μmol/L RES（P＜0.05）.The MDA content in 80 μmol/L RES was not significantly different from that in 120 μmol/L RES（P＞0.05）,extremely significantly higher than that in 0,40 μmol/L RES（P＜0.01）, significantly higher than that in 160 μmol/L（P＜0.05）.The GSH-Px activity and T-AOC in 80 μmol/L RES was the highest, the mean values were（770.21±4.54） μmol/L and（65.49±1.29） U/mg, respectively, which were extremely significantly higher than those in the 0,40,160 μmol/L RES（P＜0.01）,and were significantly higher than those in 120 μmol/L RES（P＜0.05）.The results indicated that adding 80 μmol/L RES into Tris dilution was more suitable for freezing dairy sheep sperm.