Abstract: In order to understand the genetic variation of Porcine epidemic diarrhea virus（PEDV） in part areas of Hunan Province from 2020 to 2021, 40 samples were collected from 10 pig farms with outbreaks of piglet diarrhea for PEDV nucleic acid detection; the positive rate was counted, and the S1 gene of some positive samples was amplified and sequenced; sequence homology analysis and genetic evolution analysis were carried out. The results showed that the farm positive rate was 60.0%; 21 of the 40 tissue samples were PEDV positive, and the positive rate was 52.5%. The S1 gene of PEDV strain was amplified from 6 samples which were randomly selected from 6 positive pig farms, and the target bands were all about 1 700 bp in size. The nucleotide and corresponding deduced amino acid sequence similarity were 97.2%-99.8% and 95.4%-99.5%, respectively; the corresponding sequence similarity with the popular PEDV variant（G2 type） at home and abroad was 96.8%-99.2% and 95.0%-98.7%, respectively, and the corresponding sequence similarity with PEDV vaccine strain（CV777 strain, G1 type） was 91.2%-92.3% and 88.9%-90.8%, respectively. Further genetic evolution analysis showed that the obtained 6 PEDV strains belonged to the G2 subgroup, which was very close to the branch to which the PEDV variant strains prevailed in China. The results suggested that PEDV was a common pathogen causing piglet diarrhea in part areas of Hunan Provice, and all of them were PEDV mutants.