Abstract: To understand the current status of Klebsiella pneumoniae podoconiosis serotypes, drug resistance, virulence genes and genetic evolution in Tibetan pigs, bacteria were isolated from 60 samples of fresh feces collected from Tibetan pigs with diarrhea in Linzhi and Lhasa of Tibet. Biochemical tests, 16 S rDNA gene and specific gene detection and phylogenetic tree construction were performed on the isolates. PCR method was used to detect the capsular serotype, drug resistance gene and virulence gene, and drug sensitivity test with 17 commonly used antimicrobial drugs, and the pathogenicity test was performed on mice. The results showed that three strains of Klebsiella pneumoniae of Tibetan pig origin were successfully isolated from samples from Linzhi, Tibet, with the isolation rate of 5%（3/60） and named P1, P2 and P3, respectively. The 3 isolates were able to ferment various sugars and did not produce hydrogen sulphide. The target bands of 1 400 bp and 430 bp were obtained by PCR amplification. All 3 strains clustered with CP051490 and CP064129 of Guangzhou parrot origin, with strains P1 and P2 clustered in one small cluster. The P3 strain clustered into a small cluster with CP064129, which was the most closely related. None of the three strains had pod serotypes identified. The drug resistance rate of the three strains to penicillin and ampicillin was the highest（100%）: the drug resistance rate to piracillin and erythromycin was 66.7%, followed by cephalexin and cefuroxime（33.3%）, and the three strains were sensitive to the other drugs. The three strains carried only two drug resistance genes, sul2 and blaROB-1, among which sul2+ blaROB-1 was P3, sul2 was P1, and blaROB-1 was P2. The virulence genes were uge, wabG, ureA and mrkD, respectively, with a detection rate of 28.6%. After the injection of P1 and P3 bacteria solution, the test mice showed symptoms such as increased body temperature, decreased appetite and listlessness, and all the mice died within 24 h. After P2 solution was injected, the mice showed symptoms such as increased body temperature and decreased appetite, and about 12 hours later, the mice basically recovered and did not die. The results indicated that Klebsiella pneumoniae had a certain epidemic trend in Tibetan pigs of Linzhi, Tibet.