Abstract: To improve the effect of intravenous urography in canine, low-dose（1 mL/kg, control group）, medium-dose（2 mL/kg, treatmentⅠgroup） and high-dose（3 mL/kg, treatmentⅡgroup） 60% meglumine diatrizoate were used to perform intravenous urography through forelimb veins. The angiographic effect, liver and kidney safety and urine composition were evaluated. The results showed that the contour recognition of both renal parenchyma was low in the control group and the image of the renal pelvis was not clear. There was no significant difference in liver and kidney function and urine indexes 48 hours after angiography（P＞0.05）. The images of both kidneys, renal pelvis and ureter in the treatmentⅠgroup could be clearly shown one by one 3-30 minutes after contrast examination. Comparison between 48 hours after angiography and before angiography, alanine aminotransferase（ALT）, aspartate aminotransferase（AST） activity, creatinine（CREA）, UREA contents in serum were significantly increased（P＜0.05）, urinary protein（PRO） and urobilin（BIL） in urine were significantly increased（P＜0.05 or P＜0.01）. The images of renal parenchyma, renal pelvis, ureter and bladder could be clearly shown in group Ⅱ 3-30 minutes after angiography. Compared with before angiography, ALT, CREA and UREA in serum were significantly increased 48 h after angiography（P＜0.01）, and AST was significantly increased（P＜0.05）. Urine LEU, PRO and BIL were significantly increased（P＜0.01）. The results indicated that abdominal compression method could effectively improve the imaging effect on renal parenchyma, renal pelvis, ureter and cystography. In clinical practice, the abdominal compression method combined with 2 mL/kg 60% meglumin diatrizoate had the best effect and high safety in canine intravenous urography.