Abstract: In order to establish a simple and rapid detection method of nucleic acid extraction-free fluorescence quantitative RT-PCR for Porcine reproductive and respiratory syndrome virus NADC30-like strain, the primers and probes were designed according to the NSP2 gene of the PRRSV NADC30-like strain in GenBank. A fluorescent quantitative RT-PCR method for direct detection of PRRSV NADC30-like strain in samples was established. The sensitivity, specificity, and reproducibility of the method were evaluated and tested on clinical samples. The results showed that the amplification system（20 μL） of nucleic acid extraction-free fluorescence quantitative RT-PCR method for the PRRSV NADC30-like strain was: 2×PrimeDirect Probe RT-qPCR Mix 10 μL, PRRSV NADC30-like strain solution 1 μL, NADC30-F（15 μmol/μL） 0.8 μL, NADC30-R（15 μmol/μL） 0.8 μL, NADC30-P（15 μmol/μL） 0.4 μL, sterilized purified water 7 μL. The amplification program was as follows: 90 ℃ for 3 min; 60 ℃ for 5 min; 95 ℃ for 5 s, 54 ℃ for 20 s（the fluorescence signal was collected and the FAM channel was selected）, 40 cycles. The method had good specificity and no cross-reaction to other common swine pathogens. The minimum detection limit for PRRSV NADC30-like strain solution was 3.98 TCID₅₀/100 μL, which was consistent with the sensitivity of the traditional fluorescent RT-PCR detection method for nucleic acid extraction. The intra-group and inter-group variation coefficients of samples with different virus contents were not more than 2%, with good repeatability and stability. The detection results of clinical samples were consistent with the detection results of traditional fluorescent quantitative RT-PCR method. The results indicated that the established PRRSV NADC30-like strain nucleic acid extraction-free fluorescence quantitative RT-PCR detection method had good specificity, high sensitivity, good repeatability and stability, and could be used for clinical detection of PRRSV NADC30-like strain.