Abstract: In order to determine the pathogenic bacteria that caused the suspected fowl cholera death in a chicken farm in Jingzhou City, Hubei Province, the experiment collected liver pathogen of dead chickens from the chicken farm. The diseased materials were studied by bacterial isolation and identification, 16 S rDNA cloning and sequencing analysis, PCR typing of capsule and lipopolysaccharide, drug susceptibility test and pathogenicity test. The results showed that the isolated strain was Gram-negative, and was bipolar-stained coccus by Wright’s staining. The isolated bacteria were capsule type B and lipopolysaccharide L2 by PCR typing of capsule and lipopolysaccharide, and were named as JZ-B-1. The JZ-B-1 strain had a homology of more than 99% with Pasteurella. In the phylogenetic tree, it was in the same branch with Pasteurella multocida and Pasteurella multocida subspecies, which meant that it belonged to Pasteurella multocida subspecies. The JZ-B-1 strain was resistant to piperacillin, carbenicillin, and penicillin, and was highly sensitive to ceftazidime, cefuroxime, cefradine, cefazolin, cefoperazone, ceftriaxone, ampicillin, tetracycline, neomycin, kanamycin, gentamicin, amikacin, chloramphenicol, furazolidone, vancomycin, ofloxacin, noroxacin and midecamycin. The results suggested that the strain isolated from the dead chicken was capsule B type Pasteurella multocida subspecies, which enriched the biological subtypes of chicken-derived Pasteurella multocida, and had certain guiding significance for the prevention and control of pasteurellosis and the bacterial resistance research in Hubei Province.