Abstract: In order to establish an indirect ELISA method for detecting African swine fever virus（ASFV）, the prokaryotic expression recombinant plasmid pcold-p72/p32 was constructed based on the p72/p32 gene sequence. The two recombinant plasmids were transformed into E. coli BL21（DE3） competent cells. The corresponding recombinant proteins were induced by IPTG, and the expression form of the recombinant protein was analyzed by SDS-PAGE. p72/p32 recombinant protein was purified by Ni²⁺ column affinity chromatography and was used as coating antigen. Two indirect ELISA methods for rapid detection of ASFV were established. The critical value, cross reaction, repeatability and sensitivity of the method were detected, and the clinical samples from different pig farms were verified. The results showed that the prokaryotic expression recombinant plasmid pcold-p72/p32 was successfully constructed. Both recombinant proteins could and could only react with ASFV positive serum; p32 was expressed as soluble protein and inclusion body protein, and p72 was expressed as inclusion body protein. The critical value of indirect ELISA based on p32 recombinant protein was 0.543; the critical value of indirect ELISA based on p72 recombinant protein was 0.612; the coefficients of variation of intra batch and inter batch repeatability tests were less than 10%; there was no cross reaction with the positive serum of common pathogens such as Classical swine fever virus（CSFV）, Porcine circovirus type 2（PCV-2）, Porcine pseudorabies virus（PRV）, and Porcine blue ear virus（PRRSV）; the coincidence rates with the commercial African swine fever antibody kit were 95.1% and 91.3% respectively. It showed that the two indirect ELISA methods had good specificity, sensitivity and repeatability. Through comparison, it could be preliminarily considered that the indirect ELISA method established with p32 recombinant protein as coating antigen had better detection effect, and could be used to detect ASFV antibody in pig serum samples.