Abstract: In order to study the effects of feeding amount and feeding time of chicken manure on the growth performance of black soldier fly larvae and the reduction rate and conversion rate of chicken manure, a total of 2 700 black soldier fly larvae with uniform size were randomly divided into three groups（test group Ⅰ, Ⅱ and Ⅲ） with 3 repetitions per group and 300 larvae per replicate. The amount of chicken manure per repetition per day was 48, 96,144 g, respectively. From the 10 th day after feeding chicken manure, number of larvae, insect weight and insect sand weight of each replicate were recorded regularly every day, and the survival rate of larvae, average daily gain, prepupa rate, reduction rate of chicken manure and conversion rate of chicken manure were calculated. On the 13 th day of feeding chicken feces, larvae were collected to measure and analyze routine nutrition, amino acids, fatty acids, antibiotics and feed health indexes, and insect sand and fresh chicken feces were collected to measure various indexes of organic fertilizer. The results showed that the larvae survival rate in group Ⅱ was significantly higher than that in group Ⅲ（P＜0.01）. The larval weight in group Ⅲ was significantly higher than that in group Ⅱ（P＜0.05） on the 10 th to 13 th day, and that in group Ⅱ was significantly higher than that in group Ⅰ（P＜0.01）. There was no significant difference in average daily gain among the groups（P＞0.05）. Prepupa appeared on the 11 th day after chicken manure feeding, and the prepupa rate increased significantly on the 13 th day（P＜0.01）. The reduction rate of chicken manure in group Ⅱ was significantly lower than that in group Ⅰ（P＜0.01）, and that in group Ⅲ was significantly lower than that in group Ⅱ（P＜0.01）. The conversion rate of chicken manure in group Ⅲ was significantly higher than that in group Ⅱ（P＜0.01）, and that in group Ⅱ was significantly higher than that in group Ⅰ（P＜0.01）. The crude protein content, crude fat content, calcium content, phosphorus content and total amino acid content of larvae were 46.22%, 6.98%, 8.37%, 1.16% and 45.68%, respectively. Lauric acid accounted for 33.70% of total fatty acids. There was no antibiotic residue in black soldier fly larvae, which met the requirements of the Standard of Feed Hygiene（GB 13078—2017）. Insect sand（after water volatilization） meets the Standard of Organic Fertilizer（NY 525—2012）. The results showed that under the conditions of this experiment, the optimal feeding amount of chicken manure was 96 g per 300 larvae per day, and the optimal harvest time was the 12 th day after chicken manure feeding. The larva powder could be used as high-quality protein feed, and the insect sand could be used as organic fertilizer after natural drying.