Abstract: Veterinary medicine has become the necessary input for beef cattle breeding. Whether farmers use veterinary medicine in a standardized and reasonable manner directly affects the food quality and safety of livestock and poultry products, as well as the economic benefits of beef cattle breeding. Based on the survey data of six comprehensive experimental stations in the main beef producing areas of the central plains（Henan, Shandong, Anhui and Hebei Provinces） of the national beef（yak） industrial technology system from 2019 to 2020, this study analyzed the characteristics of veterinary medicine using behavior of farmers. The influence factors of beef cattle farmers’ veterinary drug use behavior were discussed from four aspects of farmers’ personal characteristics, breeding characteristics, cognitive characteristics and external environment characteristics by using binary Logit model. The results showed that only 52.73% of the total samples were able to regulate the use of veterinary medicine, and the standardized use of veterinary medicine by sample farmers needs to be improved. In the individual characteristics of farmers, gender and age did not pass the significance test, and the education level had a significant positive impact on the standardized use of veterinary medicine at the 1% statistical level. In the breeding characteristics of farmers, the breeding years and the proportion of beef cattle breeding income in the total income failed to pass the significance test, and the breeding scale had a significant positive impact on the standardized use of veterinary medicine at the statistical level of 1%. Among the cognitive characteristics of farmers, the cognition of veterinary medicine residue harm and the cognition of drug withdrawal period had a significant positive impact on the standardized use of veterinary medicine of farmers at the statistical level of 1%, and the cognition of prohibited drug types and safe use had a significant positive impact on the standardized use of veterinary medicine of farmers at the statistical level of 5%. In the external environmental characteristics, government training had a significant positive impact on the standardized use of veterinary medicine of farmers at the statistical level of 10%, while the help from government subsidies and cooperative organizations had a significant positive impact on the standardized use of veterinary medicine of farmers at the statistical level of 1%. In view of this, the author put forward the following policy suggestions: encourage the development of large-scale and standardized livestock and poultry farms and give play to the leading role of leading breeding enterprises; strengthen the education and publicity of standardizing the use of veterinary medicine, and strengthen the training of breeding professionals; increase epidemic prevention subsidies and strengthen the supervision of veterinary medicine use.