Abstract: In order to study the influencing factors of green food enterprises’ technological innovation cooperation strategy, based on evolutionary game theory, this study constructed payment matrix and evolutionary game model; used Matlab software to conduct numerical simulation test on the evolutionary model, and carried out evolutionary game analysis on the choice of green food technology cooperation innovation strategy. The results showed that the cooperation strategy of green food enterprises was affected by the cost（I） and output（R） of the open innovation strategy carried out by both parties, the cost（C） and output（E） of the open innovation strategy used by one party alone, and the proportion of the cooperation benefit（K）. When other parameters remained unchanged, the higher I was, the higher the possibility of “open innovation, open innovation” cooperation mode was. The lower I was, the more possible that both parties would choose the cooperation mode of “closed innovation, closed innovation”. The higher R was, the more likely the two parties would choose the cooperation mode of “closed innovation, closed innovation”. The lower R was, the more likely the two parties would choose the cooperation mode of “open innovation, open innovation”. The higher C was, the more possible that the enterprise would choose the “closed innovation” strategy. The lower C was, the more likely an enterprise would choose the “open innovation” strategy. The higher E was, the more likely the enterprise would choose the “open innovation” strategy. The lower E was, the higher the possibility that the enterprise would choose the “closed innovation” strategy. When k was 0.5, both parties were most likely to choose the cooperation mode of “open innovation, open innovation”. In view of this, the green food enterprises were proposed to pay attention to the status quo of technological innovation industry and play a leading role; actively utilize external resources and cooperate with related institutions flexibly; reasonably evaluate input factors and benefit distribution, improve the initiative of cooperation.