Abstract: In order to establish a dog model of acute pancreatitis（AP）, 10 adult healthy dogs were randomly divided into two groups, the model group and the control group, with 5 dogs in each group. In the model group, a mixed solution of 5% sodium taurocholate（0.1 mL/kg by body weight） and trypsin（3 500 U/kg by body weight） was retrogradely injected into the pancreaticobiliary duct at the small duodenal papilla to establish a canine AP model; the control group was injected with the same amount of normal saline. Pain score was performed 24 hours after operation. And compared with the natural onset group（collected 5 AP natural onset dogs）, differences in clinical symptoms, imaging findings and laboratory tests were analyzed. The results showed that the model group had obvious pain response 24 hours after operation, and the pain score was（13.00±5.14） points, which was significantly higher than that of the control group（4.00±2.20） points（P＜0.05）. Dogs in both the natural onset group and the model group developed symptoms of abdominal pain, diarrhea, vomiting, loss of appetite, and lethargy; B-mode ultrasonography revealed pancreatic edema and uneven echogenicity. The hemoglobin concentration, leukocyte count, neutrophil count and hematocrit of the natural onset group were higher than the reference values, with the hemoglobin concentration significantly higher than that of the control group（P＜0.05）; the leukocyte count and neutrophil count of the model group were higher than the reference values. The differences in leukocyte count and neutrophil count among the three groups were not significant（P＞0.05）. Serum amylase, serum lipase activity and cholesterol levels were significantly or highly significantly higher in the model and natural onset groups than those in the control group（P＜0.05 or P＜0.01）; total protein levels were highly significantly lower in the model group than in the control group（P＜0.01）; blood glucose levels were highly significantly higher in the natural onset group than in the control group（P＜0.01）, and significantly lower in the model group than in the control group（P＜0.05）; all the remaining indicators in the three groups were not significantly different（P＞0.05）. The test results of pancreatic specific lipase in the model group and the natural incidence group were positive, while the control group was negative. The results suggested that the canine pancreatitis model was successfully established by surgical retrograde injection of drugs into the pancreaticobiliary duct.