Abstract: In order to find out the contamination of mycotoxins and mold in feeding corn of a dairy farm, five samples of feeding corn were collected at multiple points and multiple times. The contents of aflatoxin B₁（AFB₁）, deoxynivalenol（DON）, zearalenone（ZEN）, and ochratoxin A（OTA） in samples were tested by ELISA. The molds in the samples were isolated and identified by morphological observation, PCR amplification and phylogenetic analysis. The results showed that the detection rates of AFB₁, DON and ZEN in five samples were 100%, and OTA was not detected. Among them, AFB₁ content in one sample exceeded the national limit standard, which was 20% higher than the standard rate. DON and ZEN did not exceed the standard in all samples. After morphological observation, PCR amplification and phylogenetic analysis, three kinds of fungi were identified from the feed corn samples, namely Aspergillus flavus, Penicillium coriculatum and Fusarium proliferate. The results indicated that the feed corn of the dairy was contaminated by a variety of mycotoxins produced by Aspergillus flavus, Penicillium coriculatum and Fusarium proliferate.