Abstract: In order to explore the structure and function of Tibet yak ELOVL6 gene, the coding sequence of Tibet yak ELOVL6 gene was cloned by PCR, and the bioinformatic characteristics of its coding amino acid sequence were predicted and analyzed by online software. The expression levels of ELOVL6 gene in different tissues of Tibet yak were determined by real-time fluorescence quantitative PCR. The results showed that the coding region of Tibet yak ELOVL6 gene was 795 bp, encoding 264 amino acids. Compared with the amino acid sequence encoded by the common bovine ELOVL6 gene（GenBank accession no. NM＿001102155.1）, there was a base mutation（A→C） at the 18 th nucleotide site. Tibetan yak was closely related to Bos taurus, and it had a further relation with Danio rerio. The protein encoded by ELOVL6 gene was a hydrophobic protein that mainly performed biological functions in the endoplasmic reticulum. It had a total of 20 phosphorylation sites and a transmembrane structure with no signal peptide and a primary α-helix secondary structure. ELOVL6 gene was expressed in different tissues of Tibet yaks, and the relative expression level of ELOVL6 gene in adipose tissue was the highest and was significantly higher than that in other tissues（P＜0.01）. These results indicated that ELOVL6 gene might be a key gene regulating fatty acid metabolism in Tibet yaks.