Abstract: To explore the influence of different cultivating measures on grassland productivity in the middle of Greater Khingan Range, the test selected three pieces of sample area（Horqin Youyi Front Banner, Horqin Youyi Middle Banner and Jalaid Banner） in the middle of Greater Khingan Range, four processing, each test sample plots were set up respectively for grazing, fence enclosesure, fence enclosesure + chemical fertilizer, fence enclosesure+organic fertilize.The plot area of each treatment were 25 m~2 and three replicates were set for each treatment. At the end of the experiment, the grassland community height, vegetation coverage, and aboveground biomass（fresh grass yield and hay yield） of different treatments in each site were measured. The results showed that fence enclosure could significantly increase the height of grassland community in Horqin Youyi Front Banner and Horqin Youyi Middle Banner（P＜0.01）. In Horqin Youyi Middle Banner, fertilizer application under fence enclosure had the most obvious effect on improving the height of grassland community. However, the effects of fence closure on grassland community height in Jalaid Banner was not significant（P＞0.05）. Fence enclosure and application of organic fertilizer and chemical fertilizer under fence enclosure could improve grassland vegetation coverage of Horqin Youyi Front Banner（P＞0.05 or P＜0.05）. The application of organic fertilizer reduced the grassland vegetation coverage of fenced land in Horqin Youyi Middle Banner and Jalaid Banner（P＜0.01 or P＞0.05）. The fence enclosure could significantly improve the vegetation coverage of grassland in Horqin Youyi Middle Banner（P＜0.01）, but had no significant effect on the vegetation coverage of grassland in Jalaid Banner（P＞0.05）. Fence enclosure and application of organic fertilizer and chemical fertilizer under fence enclosure significantly or extremely significantly increased fresh grass yield in Horqin Youyi Front Banner and Horqin Youyi Middle Banner（P＜ 0.05 or P＜0.01）. The application of chemical fertilizer could significantly or extremely significantly increase the fresh grass yield of the fenced grassland in Horqin Youyi Middle Banner and Jalaid Banner（P＜0.05 or P＜0.01）, but significantly reduce the fresh grass yield of the fenced grassland in Horqin Youyi Middle Banner（P＜0.05）. The effect of fence enclosure on fresh grass yield in Jalaid Banner was not significant（P＞0.05）. The fence enclosure and the application of organic fertilizer and chemical fertilizer under the fence enclosure significantly increased the hay yield in Horqin Youyi Front Banner and Horqin Youyi Middle Banner（P＜0.01）. Fertilizer application significantly increased the hay yield of fenced land in Horqin Youyi Middle Banner and Jalaid Banner（P＜0.01）, but significantly reduced the hay yield of fenced land in Horqin Youyi Front Banner（P＜0.01）. The effect of fence closure on fresh grass yield in Jalaid Banner was not significant（P＞0.05）. In conclusion, different enclosure management measures had different effects on grassland productivity in different types of plots, and different enclosure management measures should be formulated according to the habitat characteristics of different types of grassland.