Abstract: Digital PCR（dPCR） technology is the third generation of PCR gene detection technique after conventional PCR and real-time fluorescent quantitative PCR（qPCR）. The digital PCR can achieve absolute quantitative analysis with high sensitivity and accuracy without drawing standard curves.It has the characteristics of high sensitivity and accuracy, and has been widely used in the rapid and accurate detection of animal diseases. This paper mainly introduced the evolution, principles, advantages and disadvantages of digital PCR technology, especially its application in the detection of swine diseases such as African swine fever（ASF）, porcinecirco virus disease（PCVD）, pseudorabies（PR）, porcine reproductive and respiratory syndrome（PRRS）, and brucellosis, in order to provide reference for the application of dPCR in animal disease diagnosis.