Abstract: In order to systematically investigate the role of DDX helicase family members in the growth and development of pigs, the bioinformatics method and real-time fluorescence quantitative PCR（qPCR） were used to investigate the gene structure, physical and chemical properties, protein domain, cluster evolutionary tree, GO enrichment and gene expression of 42 members of pig DDX family in the cecum of large white pigs and horse-body pigs. The results showed that all members of this family had exons and introns except for DDX28 and DDX53 genes, and the average number of exons was 16 while intron number was 15. Porcine DDX family members were distributed on all chromosomes except for chromosome 8. Most of the members of this family were unstable proteins with hydrophobic properties. Most of them had multiple subcellular localization and were mainly distributed in the nucleus. The family members predicted 15 conserved motifs, among which DDX1, DDX11, DDX19B, DDX26, DDX36, DDX39A, DDX58 and DDX60 lacked D-E-A-D conserved motifs, and the 10 conservative base sequences were found in the sequences of more than 30 members. At the same time, different members often had the same base sequence type and specific base sequence order. The 42 members of porcine DDX family were divided into 6 clusters by cluster analysis, among which DDX2 and DDX48, DDX21 and DDX50, DDX43 and DDX53, and DDX5 and DDX17 had high homology and the support rate was 100%. Most members of this family had ATP-dependent RNA helicase activity and ATP-binding function. There were 5 expression trends of DDX family genes in porcine caecum, among which DDX18, DDX21, DDX56, DDX49, DDX47, DDX20, DDX46, DDX42, DDX39B, DDX54, DDX50, DDX31 and DDX28 genes showed similar expression trends in the two cultivars. The expression of these genes was the highest at 1 day of age, while the expression level was lower at 90 and 180 day.