Abstract: The objective of this study is to screen out the virus expression vector system that can efficiently infect buffalo somatic cells and realize gene transfer. Three different viral vector-mediated transgene systems carrying enhanced green fluorescent protein（EGFP）, namely, retrovirus vector system（pMX-EGFP）, inducible lentivirus vector system（FUW-TETO-EGFP） and Lentivirus vector system（FUGW-EGFP）, were used for virus packaging. Then they infected different buffalo somatic cells（Buffalo fetal fibroblasts BFFs and buffalo stomach cells BSTC） once or twice. Fluorescence microscopy was used to observe the expression of EGFP, then flow cytometry was used to detect the infection efficiency, and finally G418 was used to screen the positive cell lines of the virus vector system with low infection efficiency. The results showed that BFFs and BSTC could be infected by the three viral vector systems, and gene transfer and expression were realized. The infection efficiency of FUGW-EGFP and FUW-TETO-EGFP was significantly higher than that of pMX-EGFP（P＜0.05）. Except for the infection of BSTC by the same type of virus carrier system, the infection efficiency of secondary infection was higher than that of primary infection, but the difference was not significant（P＞0.05）. The infection efficiency of BSTC with the same virus carrier system was higher than that of BFFs. These results indicated that FUGW-EGFP and FUW-TETO-EGFP infected buffalo somatic cells more efficiently than pMX-EGFP, and BSTC was more susceptible to virus infection than BFFs.