Abstract: To compare the three types of unconventional roughage（peanut vine, Broussonetia papyrifera and Artemisa argyi） of rumen fermentation parameters and nutrient degradation characteristics, holstein dry dairy cows with body weight of（600±30）kg equipped with permanent fistulas were selected as rumen fluid donors. The dried and crushed three kinds of unconventional roughage were put into fermentation bottles respectively, and artificial rumen fluid was added. Gas production, nutrient degradation rate（dry matter degradation rate, neutral detergent fiber degradation rate and acid detergent fiber degradation rate）, rumen fermentation parameters（pH value and volatile fatty acid content） of each ferments were measured at 2 h, 6 h, 12 h and 24 h of fermentation, respectively. The results showed that within 24 h of in vitro fermentation, the gas production of Broussonetia papyrifera rumen fermentation was significantly higher than that of peanut vine and Artemisia argyi rumen fermentation（P＜0.05）. The dry matter degradation rates, neutral detergent fiber degradation rates and acid detergent fiber degradation rates of peanut vine were higher than those of Broussonetia papyrifera and Artemisia argyi rumen fermentation（P＞0.05 or P＜0.05）. The pH values of Artemisia argyi rumen fermentation were the highest. The contents of acetic acid, propionic acid, butyric acid and total volatile acid of peanut vine rumen fermentation were the highest, while those of Artemisia argyi rumen fermentation were the lowest. It indicated that compared with Artemisia argyi, peanut vine and Broussonetia papyrifera had higher utilization value in ruminant production.