Abstract: In order to ivestigate the antigenicity of Glaesserella parasuis（HPS） outer membrane prtein（OMP） immune stimulating complexes（ISCOMs）, in this experiment, PCR method was used to amplify OMP5 gene of HPS, which was cloned into pET-28a（+） vector; the recombinant expression plasmid pET-28a-OMP5 was obtained. Furthermore, using QuilA, cholesterol and phospholipids as raw materials, dialysis method was used to prepare the HPS OMP5 antigen immune stimulating complexes（ISCOM-OMP5）, which was immunized to ICR mice for the determination of the HPS-specific IgG antibody levels, cytokines and lymphocyte proliferation effect to evaluate the immune enhancing effect of ISCOM-OMP5. The results showed that the recombinant expression plasmid was able to successfully express the target protein with a band size of about 40.0 ku; the prepared ISCOM-OMP5 had a typical honeycomb structure of ISCOM with a diameter of 30-40 nm. The encapsulation rate of OMP5 protein reached 85.8%. ISCOM-OMP5 was used for the immunological evaluation results of mice. High levels of HPS-specific IgG antibodies were detected in the serum of mice 14 days after secondary immunization. ISCOM-OMP5 could significantly increase the relative expression of interleukin 10（IL-10） and gamma interferon（IFN-γ） genes（P＜0.01）; ISCOM-OMP5 could significantly increase the proliferative capacity of T lymphocytes（P＜0.05）, and very significantly increase the proliferative capacity of T lymphocytes（P＜0.01）. The results suggested that ISCOM-OMP5 could effectively induce the immune response against HPS in mice, and could significantly enhance the immune effect.