Abstract: In order to study the effects of altitude stress on blood cortisol content, physiological and biochemical indices and blood gas indices of Marinua dogs, and to provide basic information for the introduction of police dogs at altitude, prevention and treatment of altitude diseases and prevention of altitude stress, thirty healthy Marinua dogs with similar body weight, aged 10-18 months, were transported from Kunming to Lhasa. Blood samples were collected from the dogs before transportation（before entering the plateau, the test site was in Kunming） and after arrival（after entering the plateau, the test site was in Lhasa）. The blood cortisol content, physiological indices, biochemical indices and blood gas indices were detected. The results showed that the blood cortisol content of Marinua dogs increased significantly after entering the plateau（P＜0.05）. The red blood cell count, hemoglobin content, hematocrit and lymphocyte number were significantly increased（P＜0.05）. Alkaline phosphatase activity, blood glucose concentration, urea nitrogen concentration and alanine aminotransferase activity were significantly increased（P＜0.05）, while creatinine concentration was significantly decreased（P＜0.05）. The partial pressure of carbon dioxide, total carbon dioxide and bicarbonate concentration were significantly decreased（P＜0.05）, while the concentrations of sodium ions, potassium ions and chloride ions were significantly increased（P＜0.05）. Other indices had no significant changes（P＞0.05）. These results indicated that high altitude stress could increase the blood cortisol content of Marinuar dogs, and had a great influence on some physiological and biochemical indices and blood gas indexes.