Abstract: In order to understand the drug resistance of Staphylococcus from clinical mastitis in dairy cows in Shihezi area of Xinjiang Uygur Autonomous Region, in this experiment, selective media were used to isolate Staphylococcus in milk samples from the cows with clinical mastitis in dairy farms of Shihezi area. Gram staining observation, biochemical identification, 16S rDNA gene PCR identification and drug susceptibility test were performed on the isolates. The results showed that two forms of suspicious Staphylococcus were observed by Gram staining, and one strain of Staphylococcus aureus and one strain of Staphylococcus chromogenes were isolated by biochemical identification and PCR identification of 16S rDNA gene, which were named Staphylococcus aureus sau raw milk China and Staphylococcus chromogenes sch raw milk China, respectively. Among them, Staphylococcus aureus sau raw milk China was resistant to ampicillin, gentamicin, kanamycin and cotrimoxazole, moderately resistant to penicillin G, ciprofloxacin, norfloxacin, and sensitive to cefothiophene, erythromycin, clindamycin, tetracycline and ofloxacin. Staphylococcus chromogenes sch raw milk China was moderately resistant to erythromycin and sensitive to 11 other drugs. The results indicated that Staphylococcus aureus and Staphylococcus chromogenes might be the main pathogens of clinical mastitis in dairy cows in Shihezi area, which had certain sensitivity to commonly used clinical antibiotics.