Abstract:
In order to explore the effects of different superovulation and embryo transfer treatments on the large-scale embryo transfer of Guanzhong dairy goats in different months, a total of 248 high-yield donor Guanzhong dairy ewes were divided into three batches for superovula-tion treatment in August, September and October. Each batch was divided into test group 1 and test group 2. The test group 1 was superovulated with “prostaglandin(PG)+progesterone suppository(CIDR)+follicle stimulating hormone(FSH) +luteinizing hormone releasing hormone A3(LHRH-A3)”. On the basis of the test group 1, the test group 2 was added with“pregnant horse serum gonadotropin(PMSG)”for superovula-tion. After ovulationin, the test ewes and male sheep were used for natural mating. Donor ewe embryos were collected and selected for embryo transfer. During the experiment, the average number of corpus luteum, the average number of washed embryos, the average effective embryos and the superovulation rate were measured. A total of 1 770 recipient Guanzhong dairy goats were divided into 3 batches for embryo transfer in August, September and October, and each batch was divided into test group A and test group B. The test group A was transferred by laparo-scope, and the test group B was transferred by conventional embryo surgery. Pregnancy diagnosis was conducted after embryo transfer. The number of lambs born after lambing was recorded. During the test, the operation time, the operation efficiency, the pregnancy rate and lambing rate were measured. The results showed that the average number of corpus luteum, the average number of washed embryos and the average num-ber of effective embryos in the test group 2 were extremely significantly higher than those in the test group 1(P<0. 01); the superovulation rate was higher than that of the test group 1, but the difference was not significant(P>0. 05). There was no significant difference in the average number of corpus luteum, the average number of washed eggs, the average effective embryos and the superovulation rate in August, September and October(P>0. 05), but they were the highest in September. The pregnancy rate and operation efficiency in the test group A were higher than that in the test group B, and the operation time in the group A was extremely significantly shorter than that in the test group B(P<0. 01). The pregnancy rate and lambing rate in September were the best. These results indicated that laparoscopic embryo transfer in September had the best effect by the superovulation treatment method of adding “PMSG” on the basis of “PG+CIDR+FSH+LHRH-A3” and the embryo transfer method of laparoscopic embryo transfer.