Abstract: In order to screen the interfering antibodies in clinical serum samples from animal hospitals so as to avoid false positive results in laboratory tests and which may affect clinical diagnosis, in this experiment a species-independent double antibody sandwich ELISA was used to screen and evaluate interfering antibodies in serum samples from dogs and cats received from animal hospitals in some regions of Fujian Province. Chicken IgY was used to replace purified mouse IgG to carry out interference antibody elimination test, and the correlation between the positive rates of interference antibodies and the basic characteristics of the samples was analyzed. The results showed that among 83 dog serum samples, there were 19 samples positive for interference antibodies, with a positive rate of 22.9%. Among 81 cat serum samples, there were 11 samples positive for interference antibodies, with a positive rate of 13.6%. After the interference antibody elimination test, the interference antibodies in all positive serum samples were effectively eliminated. There was no significant difference in the positive rates of interference antibodies among dogs of different ages, genders, spaying and breeds（P＞0.05）. There was significant difference in the positive rates of interference antibodies among cats of different ages（P＜0.05）, but no significant difference was found in the positive rates of interference antibodies among cats of different genders, spaying and breeds（P＞0.05）. The results suggested that there were interfering antibodies in the detected dog and cat serum samples, and replacing purified mouse IgG with chicken IgY could effectively eliminate interfering antibodies.