Abstract:
Capripoxvirus(CaPV) includes lumpy skin disease virus, goatpox virus and sheeppox virus, which can spread across borders. It has broke out or spread in most countries around the world, which has seriously affected the economic development of cattle and sheep breeding industry. Because the homology of these three viruses is as high as 96%-97%, and they do not have strict host specificity, it is difficult to distinguish CaPV members by common detection methods in terms of clinical symptoms, viral structure and antigenicity. Therefore, the establishment of an early, rapid and accurate method for identifying CaPV members will help to prevent and control the spread of the disease.The author reviewed the latest research progress in the detection of CaPV members at home and abroad, including virus isolation and identification, virus neutralization test(VNT), indirect fluorescent antibody test(IFAT), enzyme-linked immunosorbent assay(ELISA), immunoperoxidase monolayer analysis(IPMA), polymerase chain reaction(PCR), real-time fluorescence quantitative polymerase chain reaction(qPCR), loop mediated isothermal amplification test(LAMP), high resolution melting curve analysis(HRM), and recombinant polymerase amplification(RPA).The advantages and disadvantages of different detection methods were discussed in order to provide reference for further exploration of CaPV members detection methods.