Abstract: In order to explore the biological characteristics of chicken embryo-derived Salmonella in a large-scale hatchery in Taizhou area, in this experiment, Salmonella was selectively enriched and isolated from clinically dead chicken embryos infected with suspected Salmonella. Gram staining identification, specific gene amplification, virulence gene detection, drug susceptibility test and drug resistance gene detection were performed on the isolated bacteria. The results showed that a total of 6 bacteria were isolated, all of which showed small purple colonies on the chromogenic medium of Salmonella. All of them were Gram-negative bacteria, and were identified as Salmonella enterica subsp. enterica serovar Typhimurium by identification of specific gene amplification. A total of 15 virulence genes were detected from the 6 strains, except that none of them carried sopE gene; the carrying rate of sipA, sopA, sopB, ssaB, mgtC, misL, siiE, pipC, stn and fimA genes was 100%（6/6）; the carrying rate of sipC and sseL genes was 83.3%（5/6）; the carrying rate of ssaR and spvB genes was 66.7%（4/6）. The 6 strains were sensitive to most antibiotics, and only the resistance rates to doxycycline（66.7%, 4/6）, Lincomycin（100%, 6/6）, azithromycin（83.3%, 5/6） and polymycin B（83.3%, 5/6） were higher than 50.00%. The 3-fold resistance rate was 83.3%（5/6）; the 4-fold resistance rate was 66.7%（4/6）; the 5-fold resistance rate was 16.7%（1/6）, and the 6-fold resistance rate was 0（0/6）. A total of five resistance genes were detected in 6 strains, in which all detection rates of tet（A）, tet（B） and Sul2 genes were 100%（6/6）; the detection rate of bla_SHV was 83.3%（5/6）; the detection rate of Sul1 was 66.7%（4/6）; bla_KPC, bla_NDM, bla_CTX, bla_TEM, aadA1, aadB, floR, qnrA, qnrB, qnrS, mcr-1 genes were not detected. The results suggested that the 6 isolated strains of Salmonella enterica subsp. enterica serovar Typhimurium exhibited multi-drug resistance and carried a large number of virulence genes, which might be one of the important pathogens causing the death of chicken embryos in this large-scale hatchery.