Abstract: In order to evaluate the risk of parasite-derived miRNAs in extracellular membrane vesicles of Clonorchis sinensis in cross-species regulation of host genes, the miRBase database was used to screen the mature sequences of parasite-derived miRNAs shared by Clonorchis sinensis（C. sinensis） extracellular vesicles and the infected host cells. miRanda software was used to predict the target genes of the top 10 miRNAs in extracellular membrane vesicles, and the top 10 target genes of each miRNAs score were selected as candidate target genes. NCBI and Bing search tools were used to search the functions of the candidate target genes of miRNAs, and GO functional annotation and KEGG enrichment analysis were used for bioinformatics analysis of target genes. The results showed that 10 miRNAs in the extracellular vesicles of C. sinensis corresponded to a total of 8 128 host-related target genes. Twelve candidate target genes had no relevant functional reports; twelve candidate target genes were related to liver function, and the remaining candidate target genes played important roles in cancer, signal transduction, structural skeleton, proliferation and differentiation, and migration. The candidate target genes Taf10 and Onecut2 were related to the development of liver and hepatobiliary duct. Gng1 and Hhip were involved in the mitogen-activated protein kinase（MAPK） and Hedgehog signaling pathways associated with fibrosis, respectively. These results indicated that insect-derived miRNAs had the potential to target host cells and participate in the development of host liver fibrosis and liver cancer.