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杜泊羊与湖羊级进杂交试验中繁殖性能、生产性能、免疫性能及肉品质的研究

Study on reproductive performance, production performance, immune performance and meat quality of the lambs produced by grading crossing between Dorper sheep and Hu sheep

  • 摘要: 为了全面了解杜泊羊与湖羊级进杂交效果,试验选择纯种杜泊羊作为父本,分别与湖羊(杜×湖杂交组,后代记为杜湖F1代)、杜湖F1代(杜×F1代杂交组,后代记为杜湖F2代)、杜湖F2代(杜×F2代杂交组,后代记为杜湖F3代)进行杂交,以湖羊纯繁组、杜泊羊纯繁组为对照,观察各组后代羔羊体型外貌,测定各组母羊的繁殖性能(包括产羔数、产活羔数、产双羔数、产多羔数、产羔率、羔羊成活率、产双羔率、产多羔率)及后代羔羊的生产性能(羔羊初生重及1,2,3,6月龄体重和1~6月龄平均日增重,6月龄羔羊体长、体高和胸围体尺指标)、屠宰性能宰前体重、胴体重、屠宰率、眼肌面积、胴体脂肪含量(GR值)、免疫指标免疫球蛋白(Ig)A、IgM、IgG、白细胞介素(IL)和干扰素(IFN)、肉品质(pH45 min值、pH24h值、肉色、大理石纹、失水率、熟肉率、剪切力)和羊肉胴体感官评定(胴体特点、胴体重量、肥度、肉脂硬度、肌肉发育程度、肉脂色泽、适宜烹饪方式)。结果表明:随着杂交代数的增加,羔羊体型外貌明显趋向杜泊羊。杜×F1代杂交组、杜×F2代杂交组和杜泊羊纯繁组母羊的产羔率低于杜×湖杂交组和湖羊纯繁组,湖羊纯繁组的产羔率最高;杜×湖杂交组的羔羊成活率最高;湖羊纯繁组的产双羔率最高;杜×湖杂交组和湖羊纯繁组的产多羔率高于杜×F1代杂交组、杜×F2代杂交代组和杜泊羊纯繁组,杜泊羊纯繁组产双羔率为54.88%,但是产多羔率为0。随着杂交代数的增加,杜湖F1代、F2代、F3代生长速度逐渐加快,其中F3代生长速度最快,接近纯种杜泊羊,公母羔的胸围、体高和体长也不断提高。杜×F1代杂交组、杜×F2代杂交组、杜泊羊纯繁组胴体重和屠宰率显著高于湖羊纯繁组(P<0.05),各组宰前体重、眼肌面积、GR值差异不显著(P>0.05)。湖羊纯繁组血清中IgA质量浓度显著高于杜泊羊纯繁组(P<0.05),IL-2质量浓度显著高于杜×F2代杂交组和杜泊羊纯繁组(P<0.05);湖羊纯繁组和杜×湖杂交组血清中IL-6质量浓度显著高于杜泊羊纯繁组(P<0.05),各组血清中IgG、IgM、IL-1β、IL-4和IFN-γ质量浓度差异不显著(P>0.05)。各组肉品质指标均差异不显著(P>0.05),pH45 min值在6.15~6.35之间,pH24 h值在5.69~5.90之间,湖羊纯繁组大理石纹评分最高,失水率最低。湖羊纯繁组6月龄羔羊胴体偏小,膘厚、瘦肉率偏低,适合煲汤、炖煮等烹饪方式;杜×湖杂交组6月龄羔羊前胸、臀部肌肉丰满,肉脂结实,腹部脂肪呈乳白色,脂肪含量适宜,适合手抓、涮羊肉等烹饪方式;杜×F1代杂交组、杜×F2代杂交组和杜泊羊纯繁组6月龄羔羊胴体品质好,前胸、臀部肌肉丰满,瘦肉率高,肥瘦适宜,适合涮羊肉、手抓等烹饪方式。说明杜泊羊与湖羊的杂交优势明显,通过杂交可以进行商品肉羊生产,也可以培育优质肉羊新品种或新品系。

     

    Abstract: In order to comprehensively understand the effects of grading crossing between Dorper sheep and Hu sheep, in the experiment, purebred black-headed Dorper sheep were selected as the male parent to cross with Hu sheep( Dorper × Hu group and the offspring is recorded as F1 generation), F1 generation of Du × Hu sheep( Dorper ×F1 group and the offspring is recorded as F2 generation) and F2 generation of Du × Hu sheep( Dorper ×F2 group and the offspring is recorded as F3 generation), respectively, and the pure-bred Hu sheep group and the pure-bred Dorper sheep group were used as the controls. The body shape and appearance of offspring lambs in each group were observed. The reproductive performance(including number of lambs, number of live lambs, number of twin-lambs, number of multiple-lambs, lamb rate, lamb survival rate, twin-lamb rate and multiple-lamb rate), production performance(lamb birth weight, lamb weight at 1, 2, 3, and 6 months old, average daily gain from 1 to 6 months old, and body length, height and chest circumference of 6-month-old lambs), slaughter performance(pre-slaughter body weight, carcass weight, slaughtering rate, eye muscle area, and GR value), immune indexes(Ig, IL and IFN), and meat quality(pH45 min, pH24 h, meat color, marble pattern, water loss rate, cooked meat rate, and shear value) of offspring lambs and the sensory assessment indexes of mutton carcass(carcass characteristics, carcass weight, fatness, meat hardness, muscle development, meat fat color, cooking methods) were determined. The results showed that with the increase of cross breeding generations, the body shape and appearance of lambs clearly tend to be similar to those of Dorper sheep.The lambing percentage of ewes in Dorper ×F1 group, Dorper ×F2 group and pure-breeding Hu sheep group was lower than that in Dorper × Hu group and pure-breeding Hu sheep group, and the lambing percentage of pure-breeding Hu sheep group was the highest. The lamb survival rate in Dorper × Hu group was the highest. The twin rate was the highest in pure-breeding Hu sheep group. The multiple birth rate in Dorper × Hu group and pure-breeding Hu sheep group was higher than that in Dorper ×F1 group, Dorper ×F2 group and pure-breeding Hu sheep group. The twin rate in pure-breeding Dorper sheep group was 54.88%, but the multiple-lamb rate was 0. With the increase of hybrid generation, the growth rate of F1 generation, F2 generation and F3 generation generated by the cross between Dorper and Hu sheep gradually accelerated, and the growth rate of F3 generation was the fastest, which was close to the growth rate of purebred Dorper sheep. The chest circumference, body height and body length of male and female lambs were also improved. The carcass weight and slaughtering rate in Dorper ×F1 group, Dorper ×F2 group and pure-breeding Dorper sheep group were significantly higher than those in pure-breeding Hu sheep group(P<0.05), but there were no significant differences in pre-slaughter weight, eye muscle area and GR value among all groups(P> 0.05). The serum IgA mass concentration in pure-breeding Hu sheep group was significantly higher than that in pure-breeding Dorper sheep group(P<0.05), and the serum IL-2 mass concentration in pure-breeding Hu sheep group was significantly higher than that in Dorper ×F2 group and pure-breeding Dorper sheep group(P<0.05). The serum IL-6 mass concentration in pure-breeding Hu sheep group and Dorper × Hu group was significantly higher than that in pure-breeding Dorper sheep group(P<0.05), and there was no significant difference in the serum IgG, IgM, IL-1β, IL-4 and IFN-γ mass concentration among all groups(P>0.05). There was no significant difference in meat quality indexes among all groups(P>0.05). The pH45min value was between 6.15 and 6.35, and the pH24h value was between 5.69 and 5.90. The pure-breeding Hu sheep had the hightest score of marble and the lowest water loss rate. The 6-month-old lambs in pure-breeding Hu sheep group have small carcass, thick fat and lower lean meat percentage, which were suitable for cooking in soup and stew. The breast and hip muscles of the 6-month-old lambs in Dorper × Hu crossbred group were plump, the meat fat was firm. The belly fat was milky white, with fat content suitable for cooking hand-grasped mutton and instant-boiled mutton. The 6-month-old lambs in Doper×F1 group, Dorper ×F2 group and pure-breeding Dorper sheep group had good carcass quality, plunp breast and buttock muscles, and high lean meat percentage. It was suitable for instant-boiled mutton and hand-grasped mutton cooking processing. The results indicated that the crosses superiority of Dorper sheep and Hu sheep was obvious, the cross breeding could be used to produce commercial mutton sheep, and also to breed new breeds or new strains of high-quality mutton sheep.

     

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