Abstract: With the improvement of living standards, the demand of people for livestock and poultry products such as meat and milk is constantly increasing, making the breeding work of livestock and poultry particularly important. Gender control technology is an important part of accelerating livestock and poultry breeding work. Gender control technology is a kind of technology which controls the sex of offspring of livestock and poultry through human intervention to meet the market demand. At present, the field of life science is developing rapidly, and a variety of relatively mature gender control technologies have been developed, such as flow cytometry sorting method, immunological separation method, changing sperm living environment method, gene editing method, magnetic activated cell sorting（MACS） method and so on. The author summarized the above gender control technology in order to provide reference for the development of gender control technology in animal husbandry in the future.