Abstract: In order to investigate the effects of baicalin（BA） on sodium urate-induced inflammatory damage of chicken monocytes, in this experiment, monocytes were isolated from the collected chicken anticoagulant blood, and the effects of different concentrations（5, 10, 20, 40, 80, 160 μmol/L） of BA on normal chicken monocytes viability were first detected; and the BA concentration and culture time without significant effect on cell viability were selected for subsequent experiments. Cell inflammation was induced with 2 mg/mL of monosodium urate（MSU）, and cells were intervened with different concentrations of BA;the experiment contained blank control group, MSU group（2 mg/mL） and MSU + BA group with different concentrations. After a certain period of culture, nitric oxide（NO） concentration, lactate dehydrogenase（LDH） activity, the mass concentrations of interleukin-1β（IL-1β）, interleukin-18（IL-18） and tumor necrosis factor-α（TNF-α） in the cell culture supernatant were detected by the kits. The relative mRNA expression of Toll-like receptor 4（TLR4）, NOD-like receptor family domain 3（NLRP3）, nuclear factor-κB（NF-κB）, cysteine protease-1（Caspase-1）, IL-1β and IL-18 in cells were detected by real-time PCR. The results showed that the NO concentration, LDH activity, and mass concentrations of IL-1β, IL-18 and TNF-α in the model group increased significantly or very significantly（P＜0.05, or P＜0.01, or P＜0.001） compared with the blank control group; the relative expression of TLR4/NF-κB and NLRP3 signaling pathway genes mRNA was very significant（P＜0.01）. Compared with the MSU group, The NO concentration decreased significantly or very significantly in the MSU+40 μmol/L BA group and the MSU+80 μmol/L BA group（P＜0.05 or P＜0.01）;LDH viability decreased significantly in MSU+80 μmol/L BA group（P＜0.05）;the mass concentrations of IL-1β, IL-18, TNF-α in the MSU+20 μmol/L BA group and the MSU+40 μmol/L BA group decreased significantly or very significantly（P＜0.05 or P＜0.01）;except the relative expression of IL-18 mRNA in the MSU+20 μmol/L BA group was not significantly reduced（P＞0.05）, the relative mRNA expression of TLR4, NLRP3, NF-κB, Caspase-1, IL-1β, IL-18 was significantly or very significantly reduced in the MSU+10 μmol/L BA group, MSU+20 μmol/L BA group, MSU+40 μmol/L BA group and MSU+80 μmol/L BA group（P＜0.05 or P＜0.01）. The results suggested that BA could reduce MSU-induced inflammatory damage in chicken monocytes, and the mechanism might be related to down-regulation of inflammatory factor levels and inhibition of TLR4/NF-κB and NLRP3/Capase-1 signaling pathways.