Abstract: In order to study the antioxidant activity of water extract of traditional Chinese medicine compound, in this experiment, 90 healthy Kunming mice, male and female, were randomly divided into blank control group（normal saline by gavage）, positive control group（vitamin E by gavage）, low（traditional Chinese medicine compound water extract 0.4 g/mL by gavage）, medium（traditional Chinese medicine compound water extract 0.8 g/mL by gavage）, and high（traditional Chinese medicine compound water extract 1.6 g/mL by gavage） dose groups of traditional Chinese medicine compound water extract. Each group of mice were given 0.2 mL by gavage once a day for 7 days. After the experiment, the serum biochemical index, organ index, serum and liver antioxidant indexes, and immune index of each group were determined. The results showed that compared with the blank control group, there were no significant differences in serum cholesterol（CHOL）, high-density lipoprotein（HDL） and glucose（GLU） in the low, medium and high dose groups of traditional Chinese medicine compound water extracts（P＞0.05）. Triacylglycerol（TG）, creatinine（CREA）, urea nitrogen（BUN） contents and alanine aminotransferase（ALT） and alkaline phosphatase（ALP） activities were significantly reduced（P＜0.05）; the serum content of low-density lipoprotein（LDL） in the medium-dose group was significantly increased（P＜0.05）, and the activity of aspartate aminotransferase（AST） was extremely significantly decreased（P＜0.01）. The difference in heart index, liver index, spleen index, kidney index and thymus index in the low, medium and high dose groups of traditional Chinese medicine compound water extract was not significant（P＞0.05）, but all showed an upward trend. The lung indexes of the medium and high dose groups of traditional Chinese medicine compound water extract were significantly increased（P＜0.05）. Traditional Chinese medicine compound water extract could significantly increase the activities of catalase（CAT）, glutathione peroxidase（GSH-Px） and superoxide dismutase（SOD）, and total antioxidant capacity（T-AOC）（P＜0.05） in serum and liver tissues of mice, and significantly reduce the content of malondialdehyde（MDA）（P＜0.05）; the effect was more obvious with the increase of dose. The medium-dose group of traditional Chinese medicine compound water extract could significantly increase the contents of interleukin-1β（IL-1β）, IL-4, IL-6, IL-2, IL-10, immunoglobulin G（IgG）（P＜0.05）, extremely significantly increase the contents of IL-17 and immunoglobulin A（IgA）（P＜0.01） in the serum of mice. There were no significant differences in the contents of IL-1β, IL-2, IL-4, IL-10 and IL-17 in the liver of mice in the medium-dose group of traditional Chinese medicine compound water extract（P＞0.05）; the content of IL-6 was significantly reduced（P＜0.05）. The results indicated that traditional Chinese medicine compound water extract could improve the liver and kidney function of mice, improve the activities of antioxidant enzymes in serum and liver, reduce the content of MDA, and regulate the contents of immune factors in serum, thereby improved the levels of immunity and antioxidant. The effect of 0.8 g/mL was the best.