Abstract: Transcription factors are mostly expressed in cells, and are involved in the regulation of many important cellular biological processes in the body. They are indispensable components in the body. They can regulate the variable splicing process through various mechanisms and different degrees of specificity, thus promoting gene expression. Alternative splicing is closely related to the development of organisms, cell apoptosis and the occurrence of diseases, and the alternative splicing of Pre-mRNA is an important cause of the diversity of organisms’ transcriptome and proteome and can be accurately regulated by cis-regulatory elements, apparent information and other comprehensive factors. Therefore, this paper reviewed the role of transcription factors in gene alternative splicing, in order to provide a theoretical basis for the study of cell apoptosis, disease occurrence and other biological processes.