Abstract: In order to investigate the acaricidal mechanism of carvacrol and eugenol, in the experiment, firstly, three groups of carvacrol, eugenol and negative control groups were set up with three replicates in each group. Scabies mites were treated with 0.15% eugenol, 0.05% carvacrol and liquid paraffin in the carvacrol, eugenol and negative control groups, respectively, and after 4 h, scabies mite proteins were extracted. The proteins were separated by high performance liquid chromatography（HPLC） and analyzed by liquid chromatography tandem mass spectrometry（LC-MS/MS） after reductive alkylation, enzymatic digestion and tandem mass tagging（TMT）. Then the differentially expressed proteins in the carvacrol group/negative control group and the eugenol group/negative control group were analyzed, and the differentially expressed proteins were subjected to GO functional enrichment analysis and KEGG signaling pathway enrichment analysis. Finally, the differentially expressed proteins were screened for their relevance to the acaricidal effect of carvacrol and eugenol. The results showed that there were 4 692 proteins in the carvacrol group/the negative control group, of which 235 proteins were differentially expressed（113 up-regulated proteins and 122 down-regulated proteins）; there were 4 824 proteins in the eugenol group/the negative control group, of which 103 proteins were differentially expressed（43 up-regulated proteins and 60 down-regulated proteins）. The GO secondary entries of differentially expressed proteins in the carvacrol group/negative control group that were highly significantly enriched（P＜0.01） included smooth signaling pathway, regulation of protein export from the nucleus, and oxidative phosphorylation, etc.; and the GO secondary entries that were significantly enriched（P＜0.05） included the regulation of nucleoplasmic transfer, the regulation of intracellular protein transport, and glycrerolipid biosynthetic process. The GO secondary entries of differentially expressed proteins in the eugenol group/negative control group that were highly significantly enriched（P＜0.01） included tRNA modification, ncRNA processing; the GO secondary entries that were significantly enriched（P＜0.05） included cytosolic amide metabolic processes, positive regulation of protein localization to nucleus, and pteridine-containing compound catabolic process. KEGG signaling pathways that were significantly enriched（P＜0.05） for differentially expressed proteins in the carvacrol group/negative control group included alpha-linolenic acid metabolism, porphyrin and chlorophyll metabolism, and proteoglycan in cancer. KEGG signaling pathways significantly enriched（P＜0.05） in differentially expressed proteins in the eugenol group/negative control group included ribosomes, and Legionella disease. The GO secondary entry related to the mechanism of carvacrol killing mites was oxidative phosphorylation, in which phosphatidylglycerol phosphate synthetase and cytochromic C oxidase subunit 1 were annotated. Compared with negative control group, the phosphatidylglycerol phosphate synthetase in carvacrol group was significantly up-regulated（P＜0.05）, and the cytochrome C oxidase subunit 1 was significantly down-regulated（P＜0.05）. The KEGG signaling pathway related to the mechanism of eugenol killing acaroid was ribosome. The 60S subunit of ribosome L23 protein, 39S subunit of ribosome L32 protein, 39S subunit of ribosome L3 protein, 28S subunit of ribosome S16 protein and 39S subunit of ribosome L1 protein were annotated in this KEGG signaling pathway. Compared with negative control group, the above proteins in eugenol group were significantly down-regulated（P＜0.05）.The results suggested that the acaricidal mechanism of carvacrol might be related to the process of oxidative phosphorylation, and the acaricidal mechanism of eugenol might be related to the expression of ribosomal proteins.