Abstract: In order to study the effects of Chinese herbal compound additives on the serum biochemical indexes, antioxidant capacity and immune function of working dogs, 18 Labrador Retriever were randomly divided into three groups: control group, test group A and test group B, with three replicates in each group, two for each replicate（one for male and one for female）. The experiment lasted for 50 days.From the 1st to 7th days of the experiment, the animals in each group were fed with a basal diet. From the 8th day to the 50th day, the animals in the control group were fed with the basal diet, and the animals in the test group A were fed with the basal diet+5 g/kg（according to the diet weight） of Chinese herbal compound additives（prepared by Astragalus, Codonopsis pilosula, Poria cocos, Taraxacum, Lonicera japonica and Epimedium）, and the animals in the test group B was fed with the basal diet+10 g/kg（according to the diet weight） of Chinese herbal compound additives. After the test, blood was collected from forearm vein, and serum biochemical indexes（total protein TP, albumin ALB, globulin GLB mass concentration, alanine transaminase ALT, aspartate transaminase AST, alkaline phosphatase ALP, lactate dehydrogenase LDH, creatine kinase CK activity）, antioxidant indexes（Catalase CAT, total superoxide dismutase SOD, and glutathione peroxidase GSH-Px activities and malondialdehyde MDA concentration） and immune indexes（immunoglobulin A IgA, immunoglobulin G IgG, immunoglobulin M IgM mass concentration） of each group were determined. The results showed that the serum GLB mass concentration in test group A was significantly higher than that in the control group（P＜0.05）, while the serum TP and GLB mass concentrations in test group B were significantly higher than those in the control group（P＜0.05）. The serum CK activity in test group A and test group B was significantly lower than that in the control group（P＜0.01）, and there was no significant difference in other serum biochemical indicators between the test groups and the control group（P＞0.05）. There was no significant difference in all serum biochemical indicators between test group A and test group B（P＞0.05）. The serum CAT, SOD, and GSH-Px activities in test group B were significantly or extremely significantly higher than those in the control group and test group A（P＜0.05 or P＜0.01）. The serum CAT and SOD activities in test group A were significantly higher than those in the control group（P＜0.05）, and the serum MDA concentrations in test group A and test group B were significantly lower than that in the control group（P＜0.01）. The mass concentrations of serum IgA, IgG, and IgM in test group B were significantly or extremely significantly higher than those in the control group（P＜0.05 or P＜0.01）, while the mass concentrations of serum IgG in test group A were significantly higher than those in control group（P＜0.05）. There was no significant difference in the mass concentrations of IgA and IgM between the test group A and the control group（P＞0.05）. These results indicated that the addition of Chinese herbal compound additive in the diet could improve the antioxidant capacity and immune function of working dogs, and did not cause damage to the liver and kidney.Among them, the addition dose of 10 g/kg had the best effect.