Abstract: In order to investigate the biological characteristics of chicken-derived Acinetobacter indicus, in the experiment, chicken oropharyngeal swabs collected from chicken farms in Gushi County, Xinyang City, Henan Province, were carried out for the isolation and purification of Acinetobacter; the isolates were identified by Gram staining, morphological characterization, biochemical characterization, as well as analysis of the 16S rRNA gene and the zone1 and zone2 fragments of the rpoB gene; drug sensitivity test, phylogenetic analysis, nucleotide homology analysis, and pathogenicity test in mice were performed. The results showed that two bacterial isolates, named HNXY29R and HNXY36Q, were isolated from chicken oropharyngeal swab samples, which were able to grow on normal agar medium, MacConkey agar medium and blood plate, and were determined to be Gram-negative bacilli with bulbous rods or short rods by microscopic examination; and their biochemical characteristics were consistent with Acinetobacter indicus. The 16S rRNA of the two isolates had more than 99% nucleotide sequence homology with Acinetobacter indicus in GenBank; their rpoB gene zone1 and zone2 variable region fragments had more than 98% nucleotide homology with that of Acinetobacter indicus in GenBank, which further identified the two isolates as Acinetobacter indicus. Both isolates were resistant to penicillin, amoxicillin, neomycin, macromycin, kanamycin, erythromycin, ciprofloxacin, norfloxacin, rifampicin, doxycycline, cotrimoxazole, fosfenicol and tigecycline. After infecting mice with the bacterial solution at a concentration of 8.05×10~8 cfu/mL, three mice died within 24 h after infection with HNXY29R, and two mice died after infection with HNXY36Q, and no more mice died until the 7th day. The results suggested that Acinetobacter indicus was present in chickens in Gushi County, Xinyang City, Henan Province, and that the bacterium had a certain degree of pathogenicity.