Abstract: Pigeon circovirus（PiCV） is a virus found in recent years that can cause immunosuppression in infected pigeons. It belongs to the genus Circovirus of the circoviridae family. In order to study the epidemic characteristics and genetic evolution of PiCV, the whole genome sequences of 208 PiCV strains were selected from GenBank database and bioinformatics software was used in the experiment. The genetic variation of PiCV was analyzed by constructing phylogenetic tree, comparing nucleotide homology, amino acid sequence alignment, antigen epitope analysis and gene recombination. The results showed that 208 PiCV strains could be divided into two genotypes A（54.81%, 114/208） and B（45.19%, 94/208） based on the constructed phylogenetic tree, and the A genotype could be further divided into five different subpopulations A-1, A-2, A-3, A-4 and A-5. The B genotype could be further divided into four distinct subgroups: B-1, B-2, B-3 and B-4. The similarity of the whole genome sequence of 208 PiCV strains was 83.8%-100%. There were point mutation, deletion and insertion of amino acids in PiCV Cap protein. There were a large number of recombination events（44） in the whole genome sequence of 208 PiCV strains, involving the direct exchange of genetic material contained in the a genotype sequence and the transfer of small fragments of ORFC1 gene. It shows that the variation of PiCV was diverse.